Coding

Part:BBa_K942015:Design

Designed by: Luis Mario Leal Garza   Group: iGEM12_Tec-Monterrey   (2012-09-26)


SS+antiHis+Etag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 127
    Illegal BglII site found at 293
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized to be expressed in yeast. It is flanked by a secretion signal (SS) from the alpha-mating factor pre-sequence which makes the protein secretable by the yeast. An Etag was added at the C-terminal for affinity purification by L protein. This protein can be combined in fusion with another by a linker (GGGGS)4 in the C-terminal.


Source

This part is a single chain variable fragment (scFv), this is a fusion of the variable domains of antibodies, its domains come from a murine antibody.

References